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additional myoc plasmids  (OriGene)


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    OriGene additional myoc plasmids
    Additional Myoc Plasmids, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nm+000261/Myocilin+(MYOC)+(NM_000261)+Human+Tagged+ORF+Clone/pm29475084-100-0-7
    Average 90 stars, based on 5 article reviews
    additional myoc plasmids - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Binding of a glaucoma-associated myocilin variant to the αB-crystallin chaperone impedes protein clearance in trabecular meshwork cells
    Article Snippet: .. Plasmid cDNAs utilized were for WT untagged MYOC (accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_000261","term_id":"1519246545","term_text":"NM_000261"}} NM_000261 ) and CRYAB-FLAG (Origene, RC202718, accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_001885","term_id":"1676440444","term_text":"NM_001885"}} NM_001885 ), and the control vector was pCMV-Tag1. ..

    Control:

    Article Title: Binding of a glaucoma-associated myocilin variant to the αB-crystallin chaperone impedes protein clearance in trabecular meshwork cells
    Article Snippet: .. Plasmid cDNAs utilized were for WT untagged MYOC (accession no. {"type":"entrez-nucleotide","attrs":{"text":"NM_000261","term_id":"1519246545","term_text":"NM_000261"}} NM_000261 ) and CRYAB-FLAG (Origene, RC202718, accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_001885","term_id":"1676440444","term_text":"NM_001885"}} NM_001885 ), and the control vector was pCMV-Tag1. ..

    other:

    Article Title: Binding of a glaucoma-associated myocilin variant to the αB-crystallin chaperone impedes protein clearance in trabecular meshwork cells
    Article Snippet: 48 h post-transfection, the media were collected from these plates and concentrated to 100× using Ambion filter units.



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    ( A ) Western blots for human <t>MYOC</t> protein in adult transgenic mouse gastrocnemius muscle lysates. Human MYOC protein was detected in the transgenic mouse skeletal muscle and in the heart using R&D Systems anti-MYOC antibody (1:1000). Loading was 40μg tissue lysate per well of a 10% SDS-PAGE gel. Total mouse number for this representative western blot is N = 6 different animals. ( B ) NTM5 cells <t>were</t> <t>transiently-transfected</t> with a plasmid with cDNA for human MYOC-FLAG or a plasmid with cDNA for mouse Myoc-FLAG. 20μg cell lysates were treated with PNGase to remove N- glycosylation followed by Western blot analysis using anti-FLAG (1:1000). ( C ) Weights of gastrocnemius muscles and hearts harvested from equal numbers of male and female wt and CMV-MYOC-Y437H transgenic mice did not differ between groups. N per group was ≥ 6. +/- SD is indicated and t-test were p>0.1. Abbreviations–wild-type, wt; transgenic, Tg.
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    Image Search Results


    ( A ) Western blots for human MYOC protein in adult transgenic mouse gastrocnemius muscle lysates. Human MYOC protein was detected in the transgenic mouse skeletal muscle and in the heart using R&D Systems anti-MYOC antibody (1:1000). Loading was 40μg tissue lysate per well of a 10% SDS-PAGE gel. Total mouse number for this representative western blot is N = 6 different animals. ( B ) NTM5 cells were transiently-transfected with a plasmid with cDNA for human MYOC-FLAG or a plasmid with cDNA for mouse Myoc-FLAG. 20μg cell lysates were treated with PNGase to remove N- glycosylation followed by Western blot analysis using anti-FLAG (1:1000). ( C ) Weights of gastrocnemius muscles and hearts harvested from equal numbers of male and female wt and CMV-MYOC-Y437H transgenic mice did not differ between groups. N per group was ≥ 6. +/- SD is indicated and t-test were p>0.1. Abbreviations–wild-type, wt; transgenic, Tg.

    Journal: PLoS ONE

    Article Title: Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle

    doi: 10.1371/journal.pone.0206801

    Figure Lengend Snippet: ( A ) Western blots for human MYOC protein in adult transgenic mouse gastrocnemius muscle lysates. Human MYOC protein was detected in the transgenic mouse skeletal muscle and in the heart using R&D Systems anti-MYOC antibody (1:1000). Loading was 40μg tissue lysate per well of a 10% SDS-PAGE gel. Total mouse number for this representative western blot is N = 6 different animals. ( B ) NTM5 cells were transiently-transfected with a plasmid with cDNA for human MYOC-FLAG or a plasmid with cDNA for mouse Myoc-FLAG. 20μg cell lysates were treated with PNGase to remove N- glycosylation followed by Western blot analysis using anti-FLAG (1:1000). ( C ) Weights of gastrocnemius muscles and hearts harvested from equal numbers of male and female wt and CMV-MYOC-Y437H transgenic mice did not differ between groups. N per group was ≥ 6. +/- SD is indicated and t-test were p>0.1. Abbreviations–wild-type, wt; transgenic, Tg.

    Article Snippet: NTM5 cells at 70 to 80% confluence were transiently-transfected with FLAG-tagged plasmids (8μg total cDNA per 10cm plate), human MYOC (Origene, RC206556; Accession number NM_000261), or mouse Myoc (Origene, MR224777; Accession NM_010865) using FuGENE6 transfection reagent (Promega, E2691).

    Techniques: Western Blot, Transgenic Assay, SDS Page, Transfection, Plasmid Preparation, Glycoproteomics, Muscles